Respiration

Respiration

By Naomi Keddy



Respiration is the enzyme controlled release of energy from food within all living cells.
There are two Types of respiration:
1.       Aerobic Respiration – Which is the controlled release of energy from cells that requires the presence of oxygen.

Balanced equation:
C6H12O6+6O2→6CO2+6H2O+2820kJ
Glucose + Oxygen Carbon Dioxide + Water + Energy

Aerobic respiration:
 -It uses oxygen.
-It releases carbon dioxide and water.
-It releases a large amount of energy.

2. Anaerobic Respiration – Which is the controlled release of energy from cells that thrive in the absence of oxygen.

Anaerobic Respiration:
-It doesn’t require oxygen
-It releases a small amount of energy

There are two types of Anaerobic Respiration:
1. Alcohol fermentation in yeast

Equation:
Glucose Alcohol + Carbon Dioxide + Small amount of energy

2. Lactic acid fermentation

Equation:
Glucose Lactic acid + small amount of energy



The Use of Micro-Organisms in Industrial Fermentation:

Biotechnology is the use of living things or their components (cells and enzymes) to make useful products.

Bioreactors: This is the vessel in which the biological reactions take place.
Examples of Bioprocessing
Micro-organism Product Use
Bacteria Yoghurt Food
Antibiotics Kills Bacteria
Hormones Maintains health (e.g. insulin)
Yeast Ethanol Beer and Wine
Carbon Dioxide Rises Dough

.
 Stages of Aerobic Respiration:
There are three stages to be considered.
1. Glycolysis
2. Krebs Cycle
3. Electron Transport System


Stage 1: Glycolysis
Glycolysis occurs in the absence of oxygen.
-During glycolysis, glucose is broken down into two molecules of a 3-Carbon compound called pyruvic acid



Stage 2: Kreb’s Cycle
Pyruvic acid from glycolysis enters the lumen of the mitochondrion where it loses carbon dioxide to become acetyl co-enzyme A (C2)
Acetyl Co-Enzyme A enters the Kreb’s cycle.
The Kreb’s cycle is a series of chemical reactions which result in the loss of carbon dioxide and pairs of hydrogen atoms (electrons and protons).
The carbon dioxide is excreted.
The pairs of hydrogen atoms Two high energy electrons and H+ are picked up by NAD+ to form NADH which enters the membranes of the cristae of the mitochondrion where oxidative phosphorylation takes place.



Stage 3: Electron transport system:
The pairs if hydrogen atoms picked up by the NAD+ from glycolysis and the Kreb’s cycle are carried into the membranes of the cristae of the mitochondrion.
Pairs of electrons are passed down the carrier system (electron transport system) releasing energy used in phosphorylation.
Here they are passed along a system of electron carriers.
As electrons are transferred from one carrier to another enough energy is released to phosphorylate ADP to ATP three times.
At the end of the chain, the electrons and H+ are combined with oxygen to form water. 


 Glycolysis
It takes place in the cytoplasm.
-Oxygen is not used.
It takes place in aerobic and anaerobic respiration.


 Kreb’s Cycle
It takes place in the lumen of the mitochondrion.
It only occurs in aerobic respiration.

Electron transport system:
- It takes place on the cristae of the mitochondrion.
It only occurs in the presence of oxygen.
- Vitamins of the B group are required to make carriers 1 and 2
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